In-Vitro Evaluation of Phytochemical Profile and Pharmacological Potential of Methanol Derived, Natural Red Dye Extracted From Flowers of Amaryllis Belladonna

Haroon Rasheed, Faizan Ullah, Shahid Ullah Khan, Tahir Iqbal, Muhammad Hafiz Ullah Khan

Abstract


Natural extracts are frequently admired in recent times for their safer pharmacological mode. A research effort was designed and aimed to detect the bioactive phytochemical constituents and to evaluate the pharmacological potential of Amaryllis belladonna flower red dye extract (ABFRDE). The methanol derived ABFRDE, was a potent source of phytochemicals such as alkaloides (6.16%), flavonoids (0.12%), tanins (0.031%) and phenols (17.951%). The ABFRDE was tested against gram-negative (Escherichia coli) and gram-positive (Micrococcus leutus, Bacillus subtilis, Staphylococcus aureus) bacterial strains and fungal strains (Aspergillus niger, Aspergillus flavous, Aspergillus fumigatus). Highest inhibitory activity against M. luteus (6mm) &S. aureus (5mm) at 15mg/ml. M. luteus (5mm) and S. aureus (4.5mm) was achieved at 7.5 mg/ml of the ABFRDE. The inhibition in growth of all the tested bacterial strains was minimum at 1.875 mg/ml of the ABFRDE. Maximum antifungal potential against Aspergillus fumigatus, Aspergillus flavous, Aspergillu sniger was recorded at highest concentration (15mg/ml) of the ABFRDE. The free radical scavenging action of the ABFRDE was performed as % inhibition of DPPH (1, 1-diphenyl-2-picryl-hydrazyl) free radicals. The DPPH radical scavenging potential of the various concentrations of the ABFRDE can be ranked as 5 mg/10 ml > 4mg/10 ml>3mg/10 ml> 2mg/10 ml> 1mg/10 ml. We suggest further investigation of ABFRDE for identification of its active ingredients.

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International Journal of Basic Medical Sciences and Pharmacy (IJBMSP): ISSN: 2049-4963