A Validated RP-HPLC Method for the Estimation of Procyclidine Hydrochloride in Pharmaceutical Dosage Form

Abstract

A simple, rapid and precise method was developed for the quantitative estimation of procyclidine hydrochloride in pharmaceutical dosage form. A chromatographic separation of procyclidine hydrochloride was achieved with Chiral-AGP, 100 x 4.0 mm, 5.0 μm analytical column using aqueous solution of 10 mM sodium acetate pH 4.1 with acetic acid-acetonitrile (95:5 v/v). The instrumental settings include flow rate of 1.0 ml/min, column temperature at 30°C and detector wavelength of 210 nm using a photodiode array detector. Theoretical plates for procyclidine hydrochloride were 8232. Tailing factor for procyclidine hydrochloride was 1.33. The described method showed excellent linearity over a range of 2-200 g/ml for procyclidine hydrochloride. The correlation coefficient is 0.999. The relative standard deviation of peak area for six measurements is always less than 2%. Overall, the proposed method was found to be suitable and accurate for quantitative determination of procyclidine hydrochloride in pharmaceutical dosage form.