Comparative Efficacy of Aloe vera and Tamarix aphylla against Cutaneous Leishmaniasis
The pharmacological screening of methanolic extract of Aloe vera leaf and Tamarix aphylla bark were assessed to investigate the in vitro antileishmanial activity of the medicinal plants against cutaneous leishmaniasis and to develop a patent on confirmation of their activities. Different concentrations ranging from (25-100 μg/ml) of methanolic extract from Aloe vera and Tamarix aphylla were tested for their effect on the growth of leishmania promastigotes cultured in vitro from leishmania tropica inoculated in Novy-Mcnelle-Nicolle medium (NNN) and then maintained in RPMI 1640 medium. Numbers of promastigotes grown in treated culture tubes were statistically compared to the non-treated culture tubes after time interval of 48 and 96 hrs and percent growth inhibition was observed as Mean±SD in proportion to concentrations and incubation time. Maximum percent growth inhibition was observed in Aloe vera and Tamarix aphylla at concentrations of 100μg/ml in T4 (A1=66% and A2=84%) followed by T3 at 75μg/ml (B1=43% and B2=54%) after the incubation period of 96hrs respectively, while the concentration of 50μg/ml was considerable less effective in T2 (C1=27% and C2=28%) for Aloe vera and Tamarix aphylla respectively, whereas the lowest concentration of 25μg/ml fail to produce any significant effect in the parasite inhibition in both medicinal plants. In this study, Tamarix aphylla was also shown to have a statistically highly significant effect on motility of the parasites as compared of Aloe vera (P<0.05). Aloe vera and Tamarix apylla had a significant dose dependant antipromatigote activity against L. tropica as that suggest promising phytotherapeutic agents for cutaneous leishmaniasis.
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International Journal of Basic Medical Sciences and Pharmacy (IJBMSP): ISSN: 2049-4963