Evaluation of antioxidant, analgesic and antidiarrheal activity of Phoenix paludosa roxb Leaves

Sanjib Saha, Md. Khirul Islam, Md. Anisuzzman, Md. Mahadhi Hasan, Faroque Hossain, Chinmoy Talukder

Abstract


In the present study, the ethanol extract of Phoenix paludosa Roxb’s leaves were investigated for evaluating antioxidant, analgesic and antidiarrheal potential. Scavenging activity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical, total phenolic and flavonoid content and assessment of reducing power was used to investigate antioxidant potential of the leaves. Acetic acid induced writhing and hot-plate test were utilized to evaluate analgesic activity. Castor oil and magnesium sulfate induced diarrheal model and gastrointestinal motility test were used to evaluate antidiarrheal potential of the leaves. The extract showed IC50 value of 13.88 μg/ml in DPPH scavenging assay. Total phenolic content was determined by Folin-Ciocalteu’s reagent and the value was 8.29 mg GAE/g of dried plant material. Total flavonoid content was 11.42 mg QE/g of dried plant material. The extract also showed reducing power in concentration dependent manner. In dose dependent manner, the extract significantly (P < 0.001) exhibited inhibition of writhing in acetic acid induced writhing test. In hot-plate test, pain threshold was raised significantly (P < 0.001) at both doses in same manner. The extract significantly (P < 0.001) and dose dependently decreased the frequency of diarrhea and increased the latent period in both models. The extract also delayed the intestinal transit of charcoal meal in mice and the results were statistically significant (P < 0.001). The results suggest that the ethanol leaves extract of P. paludosa could be used as potential antioxidant, analgesic and antidiarrheal agent and demands further experimental analysis to clarify the underlying mechanism.

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International Journal of Basic Medical Sciences and Pharmacy (IJBMSP): ISSN: 2049-4963