Comparative Anti-Microbial Study of Different Parts of Stevia Rebaudiana Bert against Different Microorganisms

Muhammad Hussain, Sulaiman Bahadar, Malik Mujaddad Ur Rehman, Fahad Ali, Syed Wajid Ali Shah, Zarnab Zainab, Komal Habib, et al

Abstract


A major global therapeutic problem of multi drug resistant Escherichia coli and many other bacteria which produce ß-lactamase has emerged. The need to explore novel antimicrobial drugs in light of the facts of fast worldwide spread of drug resistant microbes is of supreme significance. Four types‟ extracts were prepared for each plant sample by using ethanol, acetone, hexane and water as solvents. These extract were assayed against nine human pathogenic bacterial strains and a single fungus. Antimicrobial assay was done by disc diffusion method. Leaves extract were more active than stem extracts against tested specific microbes. The highest activity among all extracts was showed by water extract of leaf against staphylococcus aurous (21-25 mm in 6 and 12 micro letter conc respectively) followed by ethanol (20-25), Acetone (25-20 mm) extracts of leaves and hexane extract of stem against Pseudomonas aeruginosa (15-22 mm) in concern concentration. This study showed that Stevia rebaudiana bert have remarkable anti-bacterial and anti-fungal activities and comparatively, leaves have high antimicrobial activity than stem

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International Journal of Basic Medical Sciences and Pharmacy (IJBMSP): ISSN: 2049-4963